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10.1038/nprot.2010.123 58 Van Der SchouwY

The sample was centrifuged at 8000g for 10 minutes at 4C and the chloroform fraction was transferred to a new tube and dried under nitrogen at 40C

But some nutrients have a more complicated story, and vitamin B12 is a fascinating example

ADO is immediately hydrolyzed into inosine and ammonia, which reacts with glutamate dehydrogenase with concomitant conversions of NADH to NAD+ measured at A340nm